UbC-StarTrack, a clonal method to target the entire progeny of individual progenitors.

摘要: Clonal cell analysis defines the potential of single cells and diversity they can produce. To achieve this, we have developed a novel adaptation genetic tracing strategy, UbC-StarTrack, which attributes specific unique color-code to neural precursors, allowing all their progeny be tracked. We used integrable fluorescent reporters driven by ubiquitous promoter in PiggyBac-based vectors inheritable stable clonal labeling. In addition, coupling this an inducible Cre-LoxP system avoids expression non-integrated reporters. assess utility system, first analyzed images combinatorial transfected progeny. also validated efficiency UbC-StarTrack trace lineages through vivo, vitro ex vivo strategies. Finally, progenitors located lateral ventricles were targeted at embryonic or postnatal stages determine neurons glia produce, relationships. way demonstrate that identify it employed wide range contexts.