Depression of amino acid transport in cultured rat hepatocytes by purified enterotoxin from Clostridiumperfringens
作者： O. Giger , M.W. Pariza
DOI: 10.1016/0006-291X(78)90620-4
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摘要: Abstract Rat liver parenchymal cells (hepatocytes) were isolated by a collagenase perfusion technique and maintained as monolayers in serum-free medium collagen-coated culture dishes. Glucagon, combination with dexamethasone, induced α-aminoisobutyric acid transport these cells. Addition of purified Clostridium perfringens enterotoxin to hepatocytes preinduced glucagon dexamethasone rapidly depressed (but did not abolish) transport. The toxin effect was dose dependent: 1000 or 300 ng/ml produced maximal depression whereas 100 40 without 120 minutes. eliminated pretreating the heat specific antisera. on two cultured rat hepatoma cell lines (H4-II-E-C3 McA-RH 7777) also investigated. Only 7777 sensitive suggesting that may interact membrane components normal which are present some all) cancerous
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