Differential discrimination of G-protein coupling of serotonin1A receptors from bovine hippocampus by an agonist and an antagonist
作者： K.G Harikumar , Amitabha Chattopadhyay
DOI: 10.1016/S0014-5793(99)01082-0
关键词:
摘要: We have studied the effect of guanosine-5′-O-(3-thiotriphosphate) (GTP-γ-S), a non-hydrolyzable analogue GTP, on agonist and antagonist binding to bovine hippocampal 5-hydroxytryptamine (5-HT)1A receptor in native membranes. Our results show that specific is inhibited with increasing concentrations GTP-γ-S along reduction affinity. In sharp contrast this, 5-HT1A shows no significant remains invariant over large range concentrations. The affinity also unaltered. This differentially discriminate G-protein coupling receptors from hippocampus.
elsevier.com
sci-hub.se 参考文章(23)
Hardy Sundaram, Adrian Newman-Tancredi, Philip G. Strange, Characterization of recombinant human serotonin 5HT1A receptors expressed in Chinese Hamster Ovary cells: [3H]spiperone discriminates between the G-protein-coupled and -uncoupled forms Biochemical Pharmacology. ,vol. 45, pp. 1003- 1009 ,(1993) , 10.1016/0006-2952(93)90243-P
Catherine D. Strader, Tung Ming Fong, Michael P. Grazlano, Michael R. Tota, The family of G-protein-coupled receptors. The FASEB Journal. ,vol. 9, pp. 745- 754 ,(1995) , 10.1096/FASEBJ.9.9.7601339
Beatriz A. Rocha, Kimberly Scearce-Levie, José J. Lucas, Noboru Hiroi, Nathalie Castanon, John C. Crabbe, Eric J. Nestler, René Hen, Increased vulnerability to cocaine in mice lacking the serotonin-1B receptor. Nature. ,vol. 393, pp. 175- 178 ,(1998) , 10.1038/30259
Mu Mu, H. F. Kung, Zhi-Ping Zhuang, Mei-Ping Kung, D. Frederick, 4-(2'-Methoxy-phenyl)-1-[2'-(n-2"-pyridinyl)-p-iodobenzamido]-ethyl- piperazine ([125I]p-MPPI) as a new selective radioligand of serotonin-1A sites in rat brain: in vitro binding and autoradiographic studies. Journal of Pharmacology and Experimental Therapeutics. ,vol. 272, pp. 429- 437 ,(1995)
B. L. Jacobs, E. C. Azmitia, Structure and function of the brain serotonin system Physiological Reviews. ,vol. 72, pp. 165- 229 ,(1992) , 10.1152/PHYSREV.1992.72.1.165
Stephen J. Peroutka, 5‐Hydroxytryptamine Receptors Journal of Neurochemistry. ,vol. 60, pp. 408- 416 ,(1993) , 10.1111/J.1471-4159.1993.TB03166.X
H. Gozlan, S. El Mestikawy, L. Pichat, J. Glowinski, M. Hamon, Identification of presynaptic serotonin autoreceptors using a new ligand: 3H-PAT. Nature. ,vol. 305, pp. 140- 142 ,(1983) , 10.1038/305140A0
A. Chattopadhyay, R. Rukmini, S. Mukherjee, Photophysics of a neurotransmitter: ionization and spectroscopic properties of serotonin Biophysical Journal. ,vol. 71, pp. 1952- 1960 ,(1996) , 10.1016/S0006-3495(96)79393-1
S. Ramboz, R. Oosting, D. A. Amara, H. F. Kung, P. Blier, M. Mendelsohn, J. J. Mann, D. Brunner, R. Hen, Serotonin receptor 1A knockout: an animal model of anxiety-related disorder. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 14476- 14481 ,(1998) , 10.1073/PNAS.95.24.14476
Francesc Artigas, Luz Romero, Claude de Montigny, Pierre Blier, Acceleration of the effect of selected antidepressant drugs in major depression by 5-HT1A antagonists Trends in Neurosciences. ,vol. 19, pp. 378- 383 ,(1996) , 10.1016/S0166-2236(96)10037-0