Validation of chromogenic in situ hybridization for detection of EGFR copy number amplification in nonsmall cell lung carcinoma.

摘要: Epidermal growth factor receptor (EGFR) gene copy number correlates with response to tyrosine kinase inhibitors in patients nonsmall cell lung carcinoma. Fluorescence situ hybridization (FISH), a standard methodology detect EGFR abnormalities carcinoma, is limited by instrumentation and cost. Chromogenic (CISH) an emerging alternative detection technique using light microscopy, but its utility assessing cancer not established. To address the of CISH, we studied paraffin-embedded carcinoma specimens from 77 Taiwanese nonsmoking women treated surgery alone. We recorded signals per tumor nucleus, correlated CISH FISH results, used receiver operating characteristics identify cut-off points for results. Tumors were classified as adenocarcinoma (n=28), mixed bronchioloalveolar features (n=25), (n=2), squamous (n=15), adenosquamous (n=7). By FISH, 29% cases had no amplification, 18% low polysomy, 35% high 12% amplification. detected highly (Spearman r=0.81, P<0.0001). determined optimal that discriminate between amplification polysomy (2.8 signals, P=0.09); plus (4.5 P<0.0001); (7.1 P=0.0003). assay determining status may contribute stratification clinical trials subset should be inhibitors.