Microsatellite analysis of small anonymous seedlot samples from pedunculate oak (Quercus robur): a promising approach to monitor the number of different seed parents and pollen donors

摘要: We have tested microsatellite analysis to monitor within-stand genetic diversity in seedlot samples from pedunculate oak (Quercus robur). focused on two key criteria of diversity: the number seed parents (mother trees) included harvests, and pollen donors (father contributing open pollinated families. Our results show that nuclear loci are suitable detect contaminations infer genotypes directly genotype data extremely small anonymous acorn (five eight acorns per mother tree), given each putative tree been supplied separately. Subsequently, different trees for such can be inferred by comparing reconstructed maternal calculating FST pairwise between Furthermore, we an approach families analysing haplotype arrays closely linked markers transmitted fathers progeny. selected three microsatellites a previously constructed map Quercus robur, then used simulated these segregating test detail. The procedures presented here designed sample sizes situations where no information is available parent population. Therefore they potentially useful monitoring commercial robur.