Detection of microsatellite instability during somatic embryogenesis of oak (Quercus robur L.)

作者: E. Wilhelm , K. Hristoforoglu , S. Fluch , K. Burg

DOI: 10.1007/S00299-004-0891-Y

关键词:

摘要: Five microsatellite loci (QpZAG1/5, QpZAG9, QpZAG36, MSQ4, MSQ13) were used to test for genetic stability of three somatic embryogenic culture lines Quercus robur L. and plantlets derived therefrom. DNA variation was detected among embryos within all lines, whereas no instability found the regenerated plants. Two revealed variation, a locus-dependent observed. The most polymorphic useful locus detecting with 28.5% investigated being variable.

参考文章(40)
H. Hirochika, K. Sugimoto, Y. Otsuki, H. Tsugawa, M. Kanda, Retrotransposons of rice involved in mutations induced by tissue culture Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 7783- 7788 ,(1996) , 10.1073/PNAS.93.15.7783
F. J. Gallego, I. Martínez, C. Celestino, M. Toribio, Testing Somaclonal Variation Using RAPDs in Quercus suber L. Somatic Embryos International Journal of Plant Sciences. ,vol. 158, pp. 563- 567 ,(1997) , 10.1086/297468
G. Hashmi, R. Huettel, R. Meyer, L. Krusberg, F. Hammerschlag, RAPD analysis of somaclonal variants derived from embryo callus cultures of peach Plant Cell Reports. ,vol. 16, pp. 624- 627 ,(1997) , 10.1007/BF01275503
Vijay Rani, S. N. Raina, Genetic fidelity of organized meristem-derived micropropagated plants: A critical reappraisal In Vitro Cellular & Developmental Biology – Plant. ,vol. 36, pp. 319- 330 ,(2000) , 10.1007/S11627-000-0059-6
P. J. Larkin, W. R. Scowcroft, Somaclonal variation — a novel source of variability from cell cultures for plant improvement Theoretical and Applied Genetics. ,vol. 60, pp. 197- 214 ,(1981) , 10.1007/BF02342540
Nathalie Isabel, Rodolphe Boivin, Caroline Levasseur, Pierre-M. Charest, Jean Bousquet, Francine M. Tremblay, OCCURRENCE OF SOMACLONAL VARIATION AMONG SOMATIC EMBRYO-DERIVED WHITE SPRUCES (PICEA GLAUCA, PINACEAE) American Journal of Botany. ,vol. 83, pp. 1121- 1130 ,(1996) , 10.1002/J.1537-2197.1996.TB13892.X
J.-L. Fourré, P. Berger, L. Niquet, P. André, Somatic embryogenesis and somaclonal variation in Norway spruce: morphogenetic, cytogenetic and molecular approaches Theoretical and Applied Genetics. ,vol. 94, pp. 159- 169 ,(1997) , 10.1007/S001220050395