Extracellular Hydrolytic Enzyme Activities of the Heterotrophic Microbial Communities of the Rouge River: An Approach to Evaluate Ecosystem Response to Urbanization

摘要: The potential effects of urbanization on the bioavailability dissolved organic carbon (DOC) were tested by determining extracellular enzyme activities heterotrophic microbial communities Rouge River. 19 enzymes monitored across two water samples (river and groundwater) at different spatial temporal scales. High phosphatase, esterase, aminopeptidase was observed in site 9 (site most exposed to anthropogenic sources) showed higher concentrations DOC compared sites 1 8 (sites less sources), where moderate diverse range observed. relative contributions overall activity as stressed increased importance peptides C source for high in-stream processing, which account nonspecific activities. In contrast, contribution glycosyl hydrolases occurred consistently all sites, highlights significance detrital plant biomass sources. Majority evidence various extents during spring summer. However, leucine aminopeptidase, valine β-glucosidase, α-mannosidase summer; alkaline phosphatase α-glucosidase spring. results presented here suggest a shift contrasting urbanization, despite similarities concentrations. Hence, API ZYM technique can be used an effective indicator river groundwater system health urban gradient.