Peripheral lymphocytes of clinically non-progressor patients harbor inactive and uninducible HIV proviruses

摘要: The HIV viral burden and RNA expression in a selected group of infected, clinically non-progressor patients were investigated. Five fast-progressor 10 AIDS cases included as controls. load was investigated by semiquantitative polymerase chain reaction (PCR) adherent macrophages genomic extragenomic fractions lymphocytes. DNA not found the subjects, weakly positive 2 5 fast-progressors strongly most patients. number proviruses lymphocytes subjects varied from to 160 copies/microgram DNA, values ten times lower than those recorded (2 LTR forms) absent or barely detectable non-progressors abundant other groups. all non-progressors. This may indicate that latent state provirus exists these subjects. To investigate this point, cultivation stimulation with PHA (phytohemoagglutinin) PMA (phorbol 12-myristate 13-acetate) attempted but after 6 days (RT-PCR for gag region) still There are no reports absence induction infected individuals. If confirmed larger patients, such non-inducibility might serve predictor marker progression disease.