MicroRNA let-7b suppresses human gastric cancer malignancy by targeting ING1

摘要: MicroRNAs (miRNAs) are important regulators that play key roles in tumorigenesis and tumor progression. In this study, we investigate whether let-7b acts as a suppressor to inhibit invasion metastasis gastric cancers. We analyzed the expression of 60 pair-matched neoplastic adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis was assessed vitro cancer cell lines with precursor inhibitor. The were using stable plasmid nude mice. A luciferase reporter assay used assess effect on inhibitor growth family, member 1 (ING1) expression. Real-time PCR showed decreased levels metastatic potentially highly metastatic. Cell migration significantly impaired GC9811-P SGC7901-M after transfection mimics. Nude mice xenograft models confirmed could vivo lentivirus pGCsil-GFP- let-7b. Luciferase assays demonstrated directly binds 3'-UTR ING1, western blotting further indicated downregulated ING1 at mRNA protein levels. Our study demonstrates overexpression can cells through targeting metastasis-associated gene ING1. These findings help clarify molecular mechanisms involved indicate modulation may be bona fide treatment cancer.