Bilayer porous scaffold based on poly-(ɛ-caprolactone) nanofibrous membrane and gelatin sponge for favoring cell proliferation

摘要: Abstract Electrospun poly-(ɛ-caprolactone) (PCL) nanofibers has been widely used in the medical prosthesis. However, poor hydrophilicity and lack of natural recognition sites for covalent cell-recognition signal molecules to promote cell attachment have limited its utility as tissue scaffolds. In this study, Bilayer porous scaffolds based on PCL electrospun membranes gelatin (GE) sponges were fabricated through soft hydrolysis followed by grafting onto fiber surface, crosslinking freeze drying treatment additional coat grafted surface. GE stably anchored membrane surface with aid molecules. The morphologies bilayer observed SEM. contact angle was 0°, mechanical properties measured tensile test, Young's moduli before after are 66–77.3 MPa 62.3–75.4 MPa, respectively. Thus, showed excellent hydrophilic desirable strength due coat. culture results that adipose derived mesenchymal stem cells did more favor adhere grow than membranes. better affinity final not only attributed chemistry but also introduction structure.