A cell culture model of the blood-brain barrier.

摘要: Endothelial cells that make up brain capillaries and constitute the blood-brain barrier become different from peripheral endothelial in response to inductive factors found nervous system. We have established a cell culture model of by treating with combination astrocyte-conditioned medium agents elevate intracellular cAMP. These form high resistance tight junctions exhibit low rates paracellular leakage fluid-phase endocytosis. They also undergo dramatic structural reorganization as they junctions. Results these studies suggest modes manipulating permeability barrier, potentially providing basis for increasing penetration drugs into central