Enhanced in vitro osteoblast differentiation on TiO2 scaffold coated with alginate hydrogel containing simvastatin.

摘要: The aim of this study was to develop a three-dimensional porous bone graft material as vehicle for simvastatin delivery and investigate its effect on primary human osteoblasts from three donors. Highly titanium dioxide (TiO2) scaffolds were submerged into containing alginate solution. Microstructure scaffolds, visualized by scanning electron microscopy micro-computed tomography, revealed an evenly distributed layer covering the surface TiO2 scaffold struts. Progressive sustained release observed up 19 days. No cytotoxic effects with when compared without simvastatin. Expression osteoblast markers (collagen type I alpha 1, alkaline phosphatase, morphogenetic protein 2, osteoprotegerin, vascular endothelial growth factor A osteocalcin) quantified using real-time reverse transcriptase–polymerase chain reaction. Secretion osteocalcin analysed multiplex immunoassay (Luminex). relative expression secretion significantly increased cells cultured 10 µM after 21 In addition, enhanced both nM at day 21. conclusion, results indicate that simvastatin-coated can support induce differentiation. combination physical properties osteogenic may represent new strategy regeneration in defects where immediate load is wanted or unavailable.