Post-transcriptional Regulation of PCSK9 by miR-191, miR-222, and miR-224.

摘要: Since proprotein convertase subtilisin kexin 9 (PCSK9) discovery, a gene involved in LDL metabolism regulation and cardiovascular diseases (CVD), many therapeutic strategies have been introduced for direct targeting of PCSK9. The main goal these has to reduce PCSK9 protein level either by application antibodies or inhibition its production. In this study, we tried discover microRNAs (miRNAs) which can target, hence regulate, expression. Using bioinformatics tools, selected three with binding sites on 3'-UTR expression miRNAs was examined different cell lines using real-time RT-PCR. We observed reciprocal pattern between miR-191, miR-222, miR-224 that Accordingly, the levels were highest Huh7 cells expressed lowest PCSK9, compared HepG2 A549 lines. mRNA also showed significant decline transfected vectors overexpressing aforementioned miRNAs. Furthermore, target cloned psiCHECK-2 vector, interaction putative investigated means luciferase assay. Our findings revealed directly interact regulate conclusion, our data introduces role