Histological Method to Study the Effect of Shear Stress on Cell Proliferation and Tissue Morphology in a Bioreactor

摘要: Tissue engineering represents a promising approach for the production of bone substitutes. The use perfusion bioreactors culture bone-forming cells on three-dimensional porous scaffold resolves mass transport limitations and provides mechanical stimuli. Despite recent important development tissue engineering, underlying mechanisms leading to substitutes remain poorly understood. In order study cell proliferation in bioreactor, we propose simplified experimental set-up using an impermeable model made 2 mm diameter glass beads which mechanosensitive cells, NIH-3T3 fibroblasts are cultured up 3 weeks under 10 mL/min medium flow. A methodology combining histological procedure, image analysis analytical calculations allows description quantification relation mean wall shear stress within bioreactor. Results show massive expansion phase after bioreactor compared static control. scenario porosity over is proposed pointing out essential role contact points between adjacent beads. Calculations indicate that experienced by changes with time, from about 50 mPa at beginning experiment 100 mPa 3 weeks. We anticipate our results will help calibration predictive models, rely estimates morphological stress.