Oral immunogenicity of the inactivated Vibrio cholerae whole-cell vaccine encapsulated in biodegradable microparticles

摘要: Abstract Vibrio cholerae (VC)-loaded microparticles as an oral vaccine delivery system were prepared with 6% w/v poly( dl -lactide-co-glycolide)(PLG) in the oil phase well 10% PVP and 5% NaCl aqueous phase, by water-in-oil-in-water emulsion/solvent extraction technique. VC was successfully entrapped trapping efficiencies up to 97.8% a loading level of 55.4±6.9 μg/mg. The microparticle particle size 3.8 μm had different distribution content core region (25.7±1.9 μg/mg) surface (6.2±0.9 μg/mg). immunogenic potential VC-loaded comparison PLG or solution evaluated adult mice immunization, which received one dose 20 mg physical mixed amphotericin B. control group solution. Serum samples collected from all tested on day immunization at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 weeks postimmunization. Sera examined for vibriocidal antibodies microtitration Vibrio-specific serum IgG IgM assessed ELISA method. intact detected sera animals immunized VC. response specific high magnitude. Significantly higher antibody responses obtained when both B titrated against immunogenicity evoking than that only. These results demonstrate orally administered evoke activity mice. incorporation, physicochemical characterization data, animal this study may be relevant optimizing incorporation properties these targeting carriers.