Histopathological alterations and induction of cytochrome P-450 1A in the liver and gills of the gilthead seabream (Sparus aurata) exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin.

摘要: The toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has been demonstrated in the seabream Sparus aurata specimens. Liver presented hepatocytic alterations, with an increase lipid droplets and glycogen granules. Ultrastructural modifications hepatocytes included RER fractionation, augmentation, as well a rise number droplets, vacuoles secondary lysosomes. In gills, lamellar epithelium showed hyperplasia, hypertrophy fusion on edge filaments. At end exposure period (1 pg l-1 TCDD for 20 days), some organelles epithelial cells lamellae tubular system chloride appeared altered. liver TCDD-exposed specimens, immunoreactive cytochrome P-450 1A was concentrated close to cytoplasmic nuclear membranes, positive granules were also evident throughout cytoplasm hepatocytes. Significant staining especially endothelium hepatic vascular system. beginning TCDD, 5 10 immunostaining observed scarce after days treatment, specific immunostained detected most gills TCDD-treated pillar-endothelial base gill filaments dispersed through lamellae. There significant branchial However, no immunoreactivity epithelial-respiratory cells.