Reference genes for qPCR assays in toxic metal and salinity stress in two flatworm model organisms

摘要: The flatworm species Schmidtea mediterranea and Macrostomum lignano have become new innovative model organisms in stem cell, regeneration tissue homeostasis research. Because of their unique cell system, (lab) technical advantages phylogenetic position within the Metazoa, they are also ideal candidate for toxicity assays. As stress biomarker screenings often performed at transcriptional level, aim this study was to establish a set reference genes qPCR experiments these two different situations. We examined stability nine potential (actb, tubb, ck2, cox4, cys, rpl13, gapdh, gm2ap, plscr1) assess those that most stable during altered conditions (exposure carcinogenic metals salinity stress). gene expression evaluated by means geNorm NormFinder algorithms. Sets best analyses varied between situations, although gm2ap actb were stably transcribed all tested combinations. In order demonstrate impact bad normalisation, stress-specific hsp90 normalised sets genes. contrast normalisation according GeNorm NormFinder, cadmium did not show significant difference when only gapdh. On other hand an increase variability noticed together. Testing appropriate is therefore strongly advisable every experimental condition.