A simple R banding technic.

作者: H A Lubs , R S Verma

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摘要: A simplified R banding technic is described which provides excellent delineation of major regions, easy identification all chromosomes, and an accurate comparison homologue lengths. The simple, requiring only initial incubation in buffer at 85 degrees C followed by acridine orange staining. best presentation the banded chromosomes was obtained printing black white from color transparency film. Variations length short arms acrocentric are clearly consistently defined. Satellites not demarcated appear as part arm. Consistent obtained, suitable for use routine clinical cytogenetic work.

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