Growth Factor-Reinforced ECM Fabricated from Chemically Hypoxic MSC Sheet with Improved In Vivo Wound Repair Activity.

摘要: MSC treatment can promote cutaneous wound repair through multiple mechanisms, and paracrine mediators secreted by are responsible for most of its therapeutic benefits. Recently, sheet composed live MSCs their ECMs was reported to healing; however, whether ECM alone could accelerate closure remained unknown. In this study, Nc-ECM Cc-ECM were prepared from nonconditioned CoCl2-conditioned sheets, respectively, healing properties evaluated in a mouse model full-thickness skin defect. Our results showed that significantly early adipocyte recruitment, rapid reepithelialization, enhanced granulation tissue growth, augmented angiogenesis. Moreover, conditioning with CoCl2 dramatically enriched collagen I, III, TGF-β1, VEGF, bFGF via activation HIF-1α hence remarkably improved ECM's vivo potency. All the Cc-ECM-treated wounds completely healed on day 7, while Nc-ECM-treated about 85.0% ± 8.6%, no-treatment only 69.8% 9.6% (p < 0.05). Therefore, we believe such growth factor-reinforced fabricated chemically hypoxic has potential clinical translation will lead MSC-derived, cost-effective, bankable biomaterial management.