Isolation of neutrophils and eosinophils from the peripheral blood of cattle and comparison of their functional activities

摘要: A procedure for isolating purified populations of neutrophils and eosinophils from bovine peripheral blood is described. The involves the centrifugation anti-coagulated whole blood, flash lysis packed red cells separation by on a Ficoll-Hypaque density gradient. following parameters were evaluated greater than 90% pure neutrophils: (1) random migration under agarose, (2) ingestion 125I-labeled Staphylococcus aureus, (3) nitroblue tetrazolium reduction, (4) iodination, or conversion 125I to trichloroacetic acid precipitable form, (5) antibody-dependent cell-mediated cytotoxicity, (6) amount intracellular peroxidase. Neutrophils significantly more active in S. aureus cytotoxicity. Eosinophils much resting stimulated iodination test they contained higher levels There was no difference between two cell types their ability reduce migrate agarose. effect granulocyte function determined. Previous contact with reduced but other tests unaffected.