Red blood cell engineering in stroma and serum/plasma-free conditions and long term storage.

摘要: In vitro generation of artificial red blood cells (RBCs) is very important to overcome insufficient and unsafe supply. Despite recent progresses in RBCs engineering from several stem cell sources, none them could succeed functional the absence serum/plasma feeder cells. Without elimination serum plasma, human RBC a large scale impossible, especially for future bioreactor system. Using an appropriate combination cost-effective safe reagents, present study demonstrated terminal maturation hematopoietic into enucleated RBCs, which were comparable donated RBCs. Surprisingly, viability erythroid was higher our serum- feeder-free culture condition than previous serum-added condition. This possible by supplementation with vitamin C media hypothermic conditions. Also, report firstly presents storability possibility essential clinical application. summary, demonstrates applicable dramatically enhanced shelf-life both stroma-free innovative technology contribute realization large-scale pharming using systems.