Complete overlap of caffeine- and K+ depolarization-sensitive intracellular calcium storage site in cultured rat arterial smooth muscle cells.
作者： S Kobayashi , H Kanaide , M Nakamura
DOI: 10.1016/S0021-9258(18)66775-4
关键词:
摘要: Changes in the concentration of cytosolic free calcium were recorded microfluorometrically rat vascular smooth muscle cells primary culture and loaded with quin-2. The effects caffeine high extracellular K+ on release from intracellular storage sites determined. In absence calcium, both depolarization plasma membrane excess application induced a transient dose-dependent elevation concentration, durations 4 2 min, respectively. Transient elevations repeatedly appeared response to repetitive (100 mM K+) (10 mM) applications progressive reductions peak levels. either case, fifth or later treatments little no rise levels calcium. amount released by after (n-1) time (1 less than equal n 5) was that n-th caffeine. exposures observed during exposure depolarization. These results indicate caffeine- depolarization-sensitive may be identical K+, optimal concentrations, will an same site cultured arterial cells, irrespective stored
sci-hub.st 参考文章(16)
J Poggioli, J P Mauger, F Guesdon, M Claret, A regulatory calcium-binding site for calcium channel in isolated rat hepatocytes. Journal of Biological Chemistry. ,vol. 260, pp. 3289- 3294 ,(1985) , 10.1016/S0021-9258(19)83619-0
A. N. Martonosi, Mechanisms of Ca2+ release from sarcoplasmic reticulum of skeletal muscle Physiological Reviews. ,vol. 64, pp. 1240- 1320 ,(1984) , 10.1152/PHYSREV.1984.64.4.1240
S Kobayashi, H Kanaide, M Nakamura, Cytosolic-free calcium transients in cultured vascular smooth muscle cells: microfluorometric measurements Science. ,vol. 229, pp. 553- 556 ,(1985) , 10.1126/SCIENCE.3927484
R. Y. Tsien, A non-disruptive technique for loading calcium buffers and indicators into cells. Nature. ,vol. 290, pp. 527- 528 ,(1981) , 10.1038/290527A0
Sei Kobayashi, Hideo Kanaide, Nakamura Motoomi, K+-depolarization induces a direct release of Ca2+ from intracellular storage sites in cultured vascular smooth muscle cells from rat aorta. Biochemical and Biophysical Research Communications. ,vol. 129, pp. 877- 884 ,(1985) , 10.1016/0006-291X(85)91973-4
David A. Williams, Kevin E. Fogarty, Roger Y. Tsien, Fredric S. Fay, Calcium gradients in single smooth muscle cells revealed by the digital imaging microscope using Fura-2 Nature. ,vol. 318, pp. 558- 561 ,(1985) , 10.1038/318558A0
Brigitte Berthon, Adrien Binet, Jean-Pierre Mauger, Michel Claret, Cytosolic free Ca2+ in isolated rat hepatocytes as measured by quin2. Effects of noradrenaline and vasopressin. FEBS Letters. ,vol. 167, pp. 19- 24 ,(1984) , 10.1016/0014-5793(84)80824-8
A. V. Somlyo, C. Franzini-Armstrong, New views of smooth muscle structure using freezing, deep-etching and rotary shadowing Cellular and Molecular Life Sciences. ,vol. 41, pp. 841- 856 ,(1985) , 10.1007/BF01970000
Hiromichi Yamamoto, Cornelis van Breemen, Inositol-1,4,5-trisphosphate releases calcium from skinned cultured smooth muscle cells Biochemical and Biophysical Research Communications. ,vol. 130, pp. 270- 274 ,(1985) , 10.1016/0006-291X(85)90412-7
Eiichi Suematsu, Masato Hirata, Toshihiko Hashimoto, Hirosi Kuriyama, Inositol 1,4,5-trisphosphate releases Ca2+ from intracellular store sites in skinned single cells of porcine coronary artery Biochemical and Biophysical Research Communications. ,vol. 120, pp. 481- 485 ,(1984) , 10.1016/0006-291X(84)91279-8