Reactive polymers in diagnostics: Syntheses and characterizations of nucleic acid probes and maleic anhydride-co-methyl vinyl ether polymers

摘要: Maleic anhydride-co-methyl vinyl ether copolymers were used as reactive polymers to link oligodeoxyribonucleotides (ODN) make oligonucleotide-copolymer conjugates of potential applications in diagnostics. The anhydride moieties the for covalent binding, via formation a peptide bond, on reaction with DNA probes that amino modified at 5′ position. best coupling yields obtained by carrying out DMSO-borate buffer (95/5 volume ratio) mixture high ionic strength. Copolymers and ODN-copolymer characterized size exclusion chromatography multiangle laser light scattering detection. average molecular weights formed relatively high, 106 g/mol range, resulting from crosslinking several copolymer molecules during coupling. Coupling onto smaller weight did not result very showing chain interpenetration was determining factor process occurs reaction. © 1997 John Wiley & Sons, Inc. J Appl Polym Sci 65: 2567–2577,