Effects of dietary vitamin E and selenium on antioxidative defense mechanisms in the liver of rats treated with high doses of glucocorticoid

摘要: The aim of this work was to determine the effects dietary intake vitamin E and selenium (Se) on lipid peroxidation as thiobarbituric acid reactive substances (TBARS) antioxidative defense mechanisms in liver rats treated with high doses prednisolone. Two hundred fifty adult male Wistar were randomly divided into five groups. fed a normal diet, but groups 3, 4, 5 received daily supplement their drinking water 20 mg E, 0.3 Se, combination respectively, for 30 d. For 3 d subsequently, control group (group 1) placebo, remaining four injected intramuscularly 100 mg/kg body weight (BW) After last administration prednisolone, 10 from each killed at 8, 12, 24, 48 h activities glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) enzymes levels (GSH) TBARS livers measured. GSH-Px, SOD, CAT enzyme GSH prednisolone-treatment 2) began decrease gradually 4 h, falling respectively 38%, 55%, 40% by 24 recovering h. In contrast, prednisolone caused an increase hepatic TBARS, reaching up times However, supplementation Se had preventive effect elevation improved diminished GSH. Therefore, present study demonstrates effectiveness reducing damage glucocorticoid-treated suggests that reductions increased result may be important factor action Se.