How the mother can help: studying maternal Wnt signaling by anti-sense-mediated depletion of maternal mRNAs and the host transfer technique.
作者： Adnan Mir , Janet Heasman
DOI: 10.1007/978-1-60327-469-2_26
关键词:
摘要: Early development in Xenopus laevis is controlled by maternal gene products synthesized during oogenesis. The dorsal/ventral and anterior/posterior axes are established as a result of canonical Wnt signaling activity. functions genes embryonic most effectively studied introducing anti-sense, oligos complementary to their mRNAs into oocytes culturing the long enough allow for breakdown target RNAs turnover existing cognate proteins before fertilization. This method has been used establish role axis formation. Here we describe methodology targeting successful fertilization mRNA-depleted oocytes.
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