Age-related differences in the expression of heat shock protein 27 by rat periodontal ligament cells in culture
作者： Kenichi Matsuzaka , Eitoyo Kokubu , Fumitoshi Ito , Shohei Ishida , Takayuki Endoh
DOI: 10.3353/OMP.14.131
关键词:
摘要: To evaluate age-related differences in the expression of heat shock protein (HSP) 27 periodontal ligament (PDL) cells at and mRNA levels, we obtained PDL from incisors rats weighing approximately 150 g (young group) 350 (aged seeded culture dishes. Over 2 weeks cultivation, were subjected to quantitative reverse transcription polymerase chain reaction (RT-PCR) measure HSP27 mRNA, was examined by immunofluorescence. young group increased slightly over weeks, while aged it significantly same time period. The ratio higher than 14 days. Furthermore, immunofluorescence signals for stronger group. These data reveal that vary their with age, suggest view such changes expression, self-defense mechanism is enhanced although overall homeostatic conditions are suppressed.
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