Effects of Hydrostatic Pressure and Transforming Growth Factor-β3 on Adult Human Mesenchymal Stem Cell Chondrogenesis In Vitro
作者： Keita Miyanishi , Michael C.D. Trindade , Derek P. Lindsey , Gary S. Beaupré , Dennis R. Carter
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摘要: This study examined the effects of intermittent hydrostatic pressure (IHP) and transforming growth factor-β3 on chondrogenesis adult human mesenchymal stem cells (hMSCs) in vitro. Chondrogenic gene expression was determined by quantifying mRNA signal levels for SOX9, a transcription factor critical cartilage development matrix proteins, aggrecan type II collagen. Extracellular production weight histology. IHP applied to hMSCs pellet culture at level 10 MPa frequency 1 Hz 4 h per day periods 3, 7, 14 days. responded addition TGF-β3 (10 ng/mL) with greater than 10-fold increase (p < 0.01) each, collagen, during 14-day period. Applying presence further increased these proteins 1.9-, 3.3-, 1.6-fold, respectively, 14. were just exposure IHP. Extracellul...
liebertonline.com参考文章(31)
H K Uhthoff, A Ooshima, Y Okawara, J Kumagai, K Sarkar, Immunohistochemical distribution of type I, II and III collagens in the rabbit supraspinatus tendon insertion. Journal of Anatomy. ,vol. 185, pp. 279- 284 ,(1994)
Qi Zhao, Heidi Eberspaecher, Véronique Lefebvre, Benoit de Crombrugghe, Parallel expression of Sox9 and Col2a1 in cells undergoing chondrogenesis. Developmental Dynamics. ,vol. 209, pp. 377- 386 ,(1997) , 10.1002/(SICI)1097-0177(199708)209:4<377::AID-AJA5>3.0.CO;2-F
Brian Johnstone, Thomas M. Hering, Arnold I. Caplan, Victor M. Goldberg, Jung U. Yoo, In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells Experimental Cell Research. ,vol. 238, pp. 265- 272 ,(1998) , 10.1006/EXCR.1997.3858
Mika Elo, Mikko J. Lammi, Reijo Sironen, Heikki J. Helminen, Kai Kaarniranta, Transcriptional activation in chondrocytes submitted to hydrostatic pressure Biorheology. ,vol. 37, pp. 85- 93 ,(2000)
LAWRENCE ROSENBERG, Chemical Basis for the Histological Use of Safranin O in the Study of Articular Cartilage Journal of Bone and Joint Surgery, American Volume. ,vol. 53, pp. 69- 82 ,(1971) , 10.2106/00004623-197153010-00007
D. J. Prockop, Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues Science. ,vol. 276, pp. 71- 74 ,(1997) , 10.1126/SCIENCE.276.5309.71
P. Das, D. J. Schurman, R. Lane Smith, Nitric oxide and G proteins mediate the response of bovine articular chondrocytes to fluid-induced shear. Journal of Orthopaedic Research. ,vol. 15, pp. 87- 93 ,(1997) , 10.1002/JOR.1100150113
Alastair M. Mackay, Stephen C. Beck, J. Mary Murphy, Frank P. Barry, Clinton O. Chichester, Mark F. Pittenger, Chondrogenic Differentiation of Cultured Human Mesenchymal Stem Cells from Marrow Tissue Engineering. ,vol. 4, pp. 415- 428 ,(1998) , 10.1089/TEN.1998.4.415
R. Lane Smith, S. F. Rusk, B. E. Ellison, P. Wessells, K. Tsuchiya, D. R. Carter, W. E. Caler, L. J. Sandell, D. J. Schurman, In vitro stimulation of articular chondrocyte mRNA and extracellular matrix synthesis by hydrostatic pressure Journal of Orthopaedic Research. ,vol. 14, pp. 53- 60 ,(1996) , 10.1002/JOR.1100140110
Allison A. Worster, Brent D. Brower-Toland, Lisa A. Fortier, Stephen J. Bent, Janice Williams, Alan J. Nixon, Chondrocytic differentiation of mesenchymal stem cells sequentially exposed to transforming growth factor-β1 in monolayer and insulin-like growth factor-I in a three-dimensional matrix Journal of Orthopaedic Research. ,vol. 19, pp. 738- 749 ,(2001) , 10.1016/S0736-0266(00)00054-1