Effect of oral dosing vehicles on the acute hepatotoxicity of carbon tetrachloride in rats

摘要: Abstract Although carbon tetrachloride (CCl 4 ) is of concern as a drinking water contaminant, it has been necessary in most oral toxicity studies to give CCl an oil vehicle due its limited solbility. The primary objective our study was assess the influence dosing vehicles on acute hepatotoxicity . Fasted 200- 230-g rats were generally found be more susceptible than fasted 300- 330-g rats. A time-course revealed that corn did not delay onset or time maximal liver injury by 100 mg/kg dose , but reduce extent relative when chemical given undiluted aqueous emulsion. male Sprague-Dawley 0, 10, 25, 50, 100, 250, 500, 1000 mg /kg body wt gavage: oil; emulsion; chemical; and 10 25 doses only, water. Blood samples taken 24 hr after for measurement serum microsomal enzymes. Pathological examination also conducted. Dose-dependent increases enzyme levels pathological changes dose-dependent decreases P450 glucose-6-phosphatase activity observed each group. Both caused significant elevations enzymes hepatic centrolobular vacuolation. less pronounced at dosage level other groups. These findings demonstrate can significantly are cause additional consideration review practice routinely using vegetable oils diluent volatile organic compound (VOC) toxicity. use Emulphor emulsions appears appropriate VOC contaminants such emulsion substantially alter from ingested