Mod5 protein binds to tRNA gene complexes and affects local transcriptional silencing

摘要: The tRNA gene-mediated (tgm) silencing of RNA polymerase II promoters is dependent on subnuclear clustering the genes, but genetic analysis shows that requires additional mechanisms. We have identified proteins bind gene transcription complexes and are required for tgm not clustering. One proteins, Mod5, a modifying enzyme adds an N6-isopentenyl adenosine modification at position 37 small number tRNAs in cytoplasm, although subpopulation Mod5 also found nucleus. Recent publications shown has tumor suppressor characteristics humans as well confers drug resistance through prion-like misfolding yeast. Here, we show associates with nucleolus. This association occurs regardless whether pre-tRNA transcripts substrates modification. In addition, bound to nuclear transcripts, they A37 Lastly, truncation transcript remove normal structure alleviates silencing, suggesting synthesis intact pre-tRNAs mechanism. These results discussed light recent showing near genes chromatin