Immunocytochemical localization of an oviductal zona pellucida glycoprotein in the oviductal epithelium of the golden hamster.

摘要: The immunocytochemical localization of an oviductal glycoprotein associated with ovulated eggs was investigated. Using a monoclonal antibody, we studied three regions epithelium in the golden hamster oviduct. antibody reacted throughout fimbriae and isthmus. Intense binding observed ampulla isthmus, especially caudal In addition, reactive materials were present ovarian bursal sac lumen ampulla. At ultrastructural level, specifically putative secretory granules Golgi apparatus nonciliated cells epithelium. Other cellular organelles did not react. Quantitative data indicated that immunolabelings intense ampullar isthmic but weak fimbrial cells. Lipid droplet-like lysosome-like vesicles isthmus react antibody. all cases, ciliated These results suggest is primarily produced secreted by then accumulated sac. findings may provide insight into regional differences secretion