Intraspecific variation in mitochondrial genome sequence, structure, and gene content in Silene vulgaris, an angiosperm with pervasive cytoplasmic male sterility.

摘要: Summary In angiosperms, mitochondrial-encoded genes can cause cytoplasmic male sterility (CMS), resulting in the coexistence of female and hermaphroditic individuals (gynodioecy). We compared four complete mitochondrial genomes from gynodioecious species Silene vulgaris found unprecedented amounts intraspecific diversity for plant DNA (mtDNA). Remarkably, only about half overall sequence content is shared between any pair genomes. The mtDNAs range size 361 to 429 kb differ gene complement, with rpl5 rps13 being intact some but absent or pseudogenized others. exhibit essentially no conservation synteny are highly repetitive, evidence reciprocal recombination occurring even across short repeats (< 250 bp). Some atypically high degrees nucleotide polymorphism, while others invariant. also contain a variable number small autonomously mapping chromosomes, which have recently been identified angiosperm mtDNA. Southern blot analysis one these chromosomes indicated complex vivo structure consisting both monomeric circles multimeric forms. We conclude that S. vulgaris harbors an unusually large degree variation mtDNA discuss extent this might be related CMS.