Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae

摘要: Mycoplasma hyopneumoniae is a prevalent swine respiratory pathogen that major cause of economic loss to pig producers. Control achieved by combination antimicrobials, vaccination and management practices, but current vaccines offer only partial control there need for improved preventative strategies. A barrier advances in understanding the pathogenesis M. developing new lack tools genetically manipulate organism. We describe development optimisation first successful plasmid-based system genetic manipulation hyopneumoniae. Our artificial plasmids contain origin replication (oriC) along with tetM, conferring resistance tetracycline. With these plasmids, we have successfully transformed strain 232 electroporation, generating tetracycline resistant organisms. The persistence extrachromosomal plasmid maintenance DNA over serial passages shows are capable self-replication In addition demonstrating amenability optimising conditions necessary transformation, used this determine minimum functional oriC doing so, developed small stably maintained multiple may be useful targeted gene disruptions. conclusion, generated set will valuable studies provide step forward study important pathogen.