Changes in protein abundance and activity involved in freezing tolerance acquisition in winter barley (Hordeum vulgare L.).

摘要: Abstract The changes in protein abundance induced by cold hardening were analysed 2 DE ten doubled haploid (DH) lines of winter barley, highly differentiated with respect to freezing tolerance level. Among 45 differential proteins identified MALDI-TOF/TOF, the majority was classified as related photosynthesis, carbohydrate metabolism, oxidation-reduction reactions and stress response. detected proteins, higher RuBisCO large small subunits, activase, two Oxygen-evolving enhancer Ferredoxin-NADP reductase, Cytochrome P450-dependent fatty acid hydroxylase 14-3-3 associated Lower relative level hypothetical ATP synthase beta subunit, uncharacterized mitochondrial AtMg00810 ribosomal RNA subunit methyltransferase G also seems be important. results proteomic studies complemented evaluation photosynthetic apparatus acclimation, showing distinctive differences between studied genotypes number active PSII reaction centres (RC/CS m ). Additionally, analysis antioxidative enzyme activities suggests importance H O a signalling molecule possibly involved initiation cold-induced plant acclimation. However, DH high tolerance, generation during treatment accompanied more stable activity catalase, -decomposing enzyme. Significance In study, two-dimensional gel electrophoresis barley. Harnessing technology resulted widening genetic variation Both cold-hardening effect on pattern an individual barley line well among selected investigated, which identification 14 metabolic pathways cellular processes. them, eight proteins: (1) precursor (2) (partial), (3) activase isoform, (4) 1-like (predicted protein), (5) 2, (6) leaf isozyme (7) M569_12509 hydroxylase-like (8) BRADI_1g11290 (14-3-3 A-like) accumulated leaves cold-hardened seedlings tolerant comparison susceptible ones. Three others: (9) BRADI_5g05668 F1 subunit-like, (10) predicted AtMg00810-like (11) BnaA02g08010D Ribosomal G-like at lower genotypes. last for first time linked complementary analyses indicate that stability enzymes under low temperature are very important acquisition.