The role of ribosomal subunits and 80-S monomers in polysome formation in an ascites tumour cell
作者： Brigid L.M. Hogan , A. Korner
DOI: 10.1016/0005-2787(68)90015-4
关键词:
摘要: The appearance of newly synthesized ribosomal subunits in 80-S monomers and polysomes has been studied Landschutz ascites tumour cells. 1. 1. Newly RNA first enters the cytoplasm as 64-S 45-S subunits. are radioactively labelled more rapidly than subunits. 2. 2. were found before they monomers. This suggests that become attached to mRNA form monomeric ribosomes. 3. 3. Cells, uniformly with [14C]uridine, transferred into non-radioactive recipient mice allowed grow for 2 4 days. During chase monomers, subunits, 50-S 30-S derived from polysomes, lost their radioactivity at approximately same rate. result shows that, during cell growth, these particles all equilibrium one another a cyclic dissociation reassociation ribosomes occurs. 4. 4. Three hypotheses concerning role protein synthesis discussed. We favour scheme which directly involved formation breakdown merely pool
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