Multiple displacement amplification

摘要: Disclosed are compositions and a method for amplification of nucleic acid sequences interest. The is based on stand displacement replication the interest by multiple primers. In one preferred form method, referred to as strand amplification, two sets primers used, right set left set. in complementary molecule be amplified opposite strand. 5' end both distal sequence when have hybridized amplified. Amplification proceeds initiated at each primer continuing through A key feature this intervening during polymerase. another whole genome random used randomly prime sample genomic (or high complexity). By choosing which sufficiently random, will collectively, randomly, distributed throughout sample. with highly processive polymerase until spontaneous termination. way, overlapping copies entire synthesized short time.