摘要: Small GTPases such as ras p21 have low intrinsic GTPase activity and depend on activating proteins (GAPs) to convent their active GTP-bound forms inactive GDP-bound counterparts (Bollag McCormick 1991b). GAPs therefore appear be major negative regulators of these (Fig. 1). In addition, we others proposed the possibility that GAP-mediated down-regulation may coupled signal output so comprise part small effector systems (Adari et al. 1988; Cales 1989; Hall 1990). This proposal is based mainly fact interact only with (a criterion for a effector), bind at or near so-called effector-binding site. The hypothesis has gained support from recent observation certain effectors heterotrimeric G-proteins are also (Berstein 1992; Arshavsky 1992), but remains controversial p21. addition issue possible role in functions GAPs, number fundamental questions relating function yet solved. For example, very little idea how, molecular level, stimulate how GAP activities regulated cells. this chapter, will discuss known properties related speculate about functions.
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sci-hub.st 参考文章(59)
M. Golubić, K. Tanaka, S. Dobrowolski, D. Wood, M.H. Tsai, M. Marshall, F. Tamanoi, D.W. Stacey, The GTPase stimulatory activities of the neurofibromatosis type 1 and the yeast IRA2 proteins are inhibited by arachidonic acid. The EMBO Journal. ,vol. 10, pp. 2897- 2903 ,(1991) , 10.1002/J.1460-2075.1991.TB07839.X
S Park, M.S. Marshall, J.B. Gibbs, R Jove, Reconstitution of interactions between the Src tyrosine kinases and Ras GTPase-activating protein using a baculovirus expression system. Journal of Biological Chemistry. ,vol. 267, pp. 11612- 11618 ,(1992) , 10.1016/S0021-9258(19)49955-9
M R Mitts, J Bradshaw-Rouse, W Heideman, Interactions between adenylate cyclase and the yeast GTPase-activating protein IRA1. Molecular and Cellular Biology. ,vol. 11, pp. 4591- 4598 ,(1991) , 10.1128/MCB.11.9.4591
P Gideon, J John, M Frech, A Lautwein, R Clark, J E Scheffler, A Wittinghofer, MUTATIONAL AND KINETIC ANALYSES OF THE GTPASE-ACTIVATING PROTEIN (GAP)-P21 INTERACTION - THE C-TERMINAL DOMAIN OF GAP IS NOT SUFFICIENT FOR FULL ACTIVITY Molecular and Cellular Biology. ,vol. 12, pp. 2050- 2056 ,(1992) , 10.1128/MCB.12.5.2050
J B Gibbs, M S Marshall, The ras oncogene--an important regulatory element in lower eucaryotic organisms. Microbiological Research. ,vol. 53, pp. 171- 185 ,(1989) , 10.1128/MR.53.2.171-185.1989
A. Kashishian, A. Kazlauskas, J.A. Cooper, Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and PI3 kinase in vivo. The EMBO Journal. ,vol. 11, pp. 1373- 1382 ,(1992) , 10.1002/J.1460-2075.1992.TB05182.X
K Tanaka, M Nakafuku, F Tamanoi, Y Kaziro, K Matsumoto, A Toh-e, IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein. Molecular and Cellular Biology. ,vol. 10, pp. 4303- 4313 ,(1990) , 10.1128/MCB.10.8.4303
K Tanaka, K Matsumoto, A Toh-E, IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae. Molecular and Cellular Biology. ,vol. 9, pp. 757- 768 ,(1989) , 10.1128/MCB.9.2.757
M H Tsai, A Hall, D W Stacey, Inhibition by phospholipids of the interaction between R-ras, rho, and their GTPase-activating proteins. Molecular and Cellular Biology. ,vol. 9, pp. 5260- 5264 ,(1989) , 10.1128/MCB.9.11.5260
M Frech, J John, V Pizon, P Chardin, A Tavitian, R Clark, F McCormick, A Wittinghofer, Inhibition of GTPase activating protein stimulation of Ras-p21 GTPase by the Krev-1 gene product Science. ,vol. 249, pp. 169- 171 ,(1990) , 10.1126/SCIENCE.2164710