Species delimitation and genetic structure of two endemic Magnolia species (section Magnolia; Magnoliaceae) in Mexico.

摘要: Magnolias are characteristic tree species of the Tropical Montane Cloud Forest (TMCF) in Mexico, an ecosystem that is highly threatened by habitat fragmentation and climate change. In this study, based on DNA sequences from five regions (chloroplast: trnT-trnL, trnK5-matK, trnS-trnG, rpl32-trnL, nuclear: ITS) seven nuclear microsatellite markers, we aimed to delineate boundaries between two-endemic TMCF, Magnolia pedrazae schiedeana, estimate levels genetic structure diversity among populations. Phylogenetic haplotype network analyses for chloroplast ITS did not support differentiation as two distinctive species. Results Bayesian multivariate cluster loci showed high across most populations, which was consistent with a strong significant pattern isolation geographical distance. We found moderate population diversity, but it lower small populations relative large Our results suggest contemporary decrease connectivity likely consequence current decline suitable TMCF habitat. Managing landscape remnant within protected natural parks surroundings, emphasis would be key conservation.