Heparin nanogel-containing liposomes for intracellular RNase delivery
作者： Dai Hai Nguyen , Jung Seok Lee , Jong Hoon Choi , Yunki Lee , Joo Young Son
DOI: 10.1007/S13233-015-3093-2
关键词:
摘要: Liposomes containing nanogels (liponanogels) were fabricated by sonicating heparin-Pluronic (HP) with pegylated lipids for ribonuclease (RNase) delivery. Liponanogels an average diameter of 316 nm obtained and their spherical morphology was elucidated atomic force microscopy (AFM). Confocal laser scanning (CLSM) revealed the core-shell structure liponanogels using two different fluorescent dyes, showing that HP localized in core liposomes. Interestingly, hybridization these systems remedies drawbacks each system while they hold strengths called “WIN-WIN effect”. When used to encapsulate RNase liponanogels, loading almost doubled as compared liposomes without nanogels. Due presence a lipid bilayer on nanogels, release prolonged over 4 days whereas it much faster (82% after 21 h) bare The cytotoxicity RNase-loaded higher than free because endocytic cellular uptake particles. We believe hybrid liponanogel can potentially be utilized hereditary diseases targeted cancer therapy since efficiently load RNases sustainly target cells. Open image new window
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