Prospective isolation of human erythroid lineage-committed progenitors

摘要: Determining the developmental pathway leading to erythrocytes and being able isolate their progenitors are crucial understanding treating disorders of red cell imbalance such as anemia, myelodysplastic syndrome, polycythemia vera. Here we show that human erythrocyte progenitor (hEP) can be prospectively isolated from adult bone marrow. We found three subfractions possessed different expression patterns CD105 CD71 within previously defined megakaryocyte/erythrocyte (hMEP; Lineage− CD34+ CD38+ IL-3Rα− CD45RA−) population. Both CD71− CD105− CD71+ MEPs, at least in vitro, still retained bipotency for megakaryocyte (MegK) (E) lineages, although latter subpopulation is skewed differentiation toward erythroid lineage. Notably, proliferative output CD71intermediate(int)/+ CD105+ subset cells MEP population was completely restricted lineage with loss MegK potential. MEPs erythrocyte-biased (E-MEPs) CD71int/+ EPs. These unclassified populations may facilitate further molecular mechanisms governing development serve potential therapeutic targets