Plasma Circulating Tumor DNA in Pancreatic Cancer Patients Is a Prognostic Marker.

作者: Daniel Pietrasz , Nicolas Pécuchet , Fanny Garlan , Audrey Didelot , Olivier Dubreuil

DOI: 10.1158/1078-0432.CCR-16-0806

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摘要: Purpose: Despite recent therapeutic advances, prognosis of patients with pancreatic adenocarcinoma remains poor. Analyses from tumor tissues present limitations; identification informative marker blood might be a promising alternative. The aim this study was to assess the feasibility and prognostic value circulating DNA (ctDNA) in adenocarcinoma. Experimental Design: From 2011 2015, samples were prospectively collected all consecutive treated our center. Identification ctDNA done next-generation sequencing targeted on referenced mutations picoliter droplet digital PCR. Results: A total 135 resectable (n = 31; 23%), locally advanced 36; 27%), or metastatic 68; 50%) included. In 104), 48% 50) had detectable median mutation allelic frequency (MAF) 6.1%. presence strongly correlated poor overall survival (OS; 6.5 vs. 19.0 months; P Conclusions: is an independent Furthermore, it arises as indicator shorter disease-free resected when detected after surgery. Clin Cancer Res; 23(1); 116–23. ©2016 AACR.

参考文章(29)
Mark Sausen, Jillian Phallen, Vilmos Adleff, Siân Jones, Rebecca J Leary, Michael T Barrett, Valsamo Anagnostou, Sonya Parpart-Li, Derek Murphy, Qing Kay Li, Carolyn A Hruban, Rob Scharpf, James R White, Peter J O’Dwyer, Peter J Allen, James R Eshleman, Craig B Thompson, David S Klimstra, David C Linehan, Anirban Maitra, Ralph H Hruban, Luis A Diaz Jr, Daniel D Von Hoff, Julia S Johansen, Jeffrey A Drebin, Victor E Velculescu, None, Clinical implications of genomic alterations in the tumour and circulation of pancreatic cancer patients Nature Communications. ,vol. 6, pp. 7686- 7686 ,(2015) , 10.1038/NCOMMS8686
Christie Eheman, S. Jane Henley, Rachel Ballard-Barbash, Eric J. Jacobs, Maria J. Schymura, Anne-Michelle Noone, Liping Pan, Robert N. Anderson, Janet E. Fulton, Betsy A. Kohler, Ahmedin Jemal, Elizabeth Ward, Marcus Plescia, Lynn A. G. Ries, Brenda K. Edwards, Annual Report to the Nation on the status of cancer, 1975-2008, featuring cancers associated with excess weight and lack of sufficient physical activity. Cancer. ,vol. 118, pp. 2338- 2366 ,(2012) , 10.1002/CNCR.27514
Ralph H. Hruban, Jason Y. Park, Seung Mo Hong, Michael Goggins, Molecular Signatures of Pancreatic Cancer Archives of Pathology & Laboratory Medicine. ,vol. 135, pp. 716- 727 ,(2011) , 10.1043/2010-0566-RA.1
Hideaki Kinugasa, Kazuhiro Nouso, Koji Miyahara, Yuki Morimoto, Chihiro Dohi, Koichiro Tsutsumi, Hironari Kato, Takehiro Matsubara, Hiroyuki Okada, Kazuhide Yamamoto, Detection of K-ras gene mutation by liquid biopsy in patients with pancreatic cancer. Cancer. ,vol. 121, pp. 2271- 2280 ,(2015) , 10.1002/CNCR.29364
Nicola Waddell, Marina Pajic, Ann-Marie Patch, David K Chang, Karin S Kassahn, Peter Bailey, Amber L Johns, David Miller, Katia Nones, Kelly Quek, Michael CJ Quinn, Alan J Robertson, Muhammad ZH Fadlullah, Tim JC Bruxner, Angelika N Christ, Ivon Harliwong, Senel Idrisoglu, Suzanne Manning, Craig Nourse, Ehsan Nourbakhsh, Shivangi Wani, Peter J Wilson, Emma Markham, Nicole Cloonan, Matthew J Anderson, J Lynn Fink, Oliver Holmes, Stephen H Kazakoff, Conrad Leonard, Felicity Newell, Barsha Poudel, Sarah Song, Darrin Taylor, Nick Waddell, Scott Wood, Qinying Xu, Jianmin Wu, Mark Pinese, Mark J Cowley, Hong C Lee, Marc D Jones, Adnan M Nagrial, Jeremy Humphris, Lorraine A Chantrill, Venessa Chin, Angela M Steinmann, Amanda Mawson, Emily S Humphrey, Emily K Colvin, Angela Chou, Christopher J Scarlett, Andreia V Pinho, Marc Giry-Laterriere, Ilse Rooman, Jaswinder S Samra, James G Kench, Jessica A Pettitt, Neil D Merrett, Christopher Toon, Krishna Epari, Nam Q Nguyen, Andrew Barbour, Nikolajs Zeps, Nigel B Jamieson, Janet S Graham, Simone P Niclou, Rolf Bjerkvig, Robert Grützmann, Daniela Aust, Ralph H Hruban, Anirban Maitra, Christine A Iacobuzio-Donahue, Christopher L Wolfgang, Richard A Morgan, Rita T Lawlor, Vincenzo Corbo, Claudio Bassi, Massimo Falconi, Giuseppe Zamboni, Giampaolo Tortora, Margaret A Tempero, Royal North Shore Hospital Samra Jaswinder S. 39 Gill Anthony J. 39 Pavlakis Nick 39 Guminski Alex 39 Toon Christopher 39, Bankstown Hospital Asghari Ray 40 Merrett Neil D. 40 Pavey Darren 40 Das Amitabha 40, Liverpool Hospital Cosman Peter H. 41 Ismail Kasim 41 O’Connnor Chelsie 41, Westmead Hospital Lam Duncan McLeod Vincent W. 42 42 Pleass Henry C. 42 Richardson Arthur 42 James Virginia 42, Royal Prince Alfred Hospital Kench James G. 43 Cooper Caroline L. 43 Joseph David 43 Sandroussi Charbel 43 Crawford Michael 43 Gallagher James 43, Fremantle Hospital Texler Michael 44 Forest Cindy 44 Laycock Andrew 44 Epari Krishna P. 44 Ballal Mo 44 Fletcher David R. 44 Mukhedkar Sanjay 44, Sir Charles Gairdner Hospital Spry Nigel A. 45 DeBoer Bastiaan 45 Chai Ming 45, St John of God Healthcare Zeps Nikolajs 46 Beilin Maria 46 Feeney Kynan 46, Royal Adelaide Hospital Nguyen Nan Q. 47 Ruszkiewicz Andrew R. 47 Worthley Chris 47 Tan Chuan P. 47 Debrencini Tamara 47, Flinders Medical Centre Chen John 48 Brooke-Smith Mark E. 48 Papangelis Virginia 48, Greenslopes Private Hospital Tang Henry 49 Barbour Andrew P. 49, Envoi Pathology Clouston Andrew D. 50 Martin Patrick 50, Princess Alexandria Hospital O’Rourke Thomas J. 51 Chiang Amy 51 Fawcett Jonathan W. 51 Slater Kellee 51 Yeung Shinn 51 Hatzifotis Michael 51 Hodgkinson Peter 51, Austin Hospital Christophi Christopher 52 Nikfarjam Mehrdad 52 Mountain Angela 52, Victorian Cancer Biobank, Johns Hopkins Medical Institutes Eshleman James R. 54 Hruban Ralph H. 54 Maitra Anirban 54 Iacobuzio-Donahue Christine A. 54 Schulick Richard D. 54 Wolfgang Christopher L. 54 Morgan Richard A 54 Hodgin Mary 54, ARC-Net Centre for Applied Research on Cancer Scarpa Aldo 55 Lawlor Rita T. 55 Beghelli Stefania 55 Corbo Vincenzo 55 Scardoni Maria 55 Bassi Claudio 55, University of California, San Francisco Tempero Margaret A. 56, University of Glasgow Biankin Andrew V. 36 37 57 Grimmond Sean M. 37 38 Chang David K. 36 37 57 Musgrove Elizabeth A. 37 Jones Marc D. 36 37 Nourse Craig 37 38 Jamieson Nigel B. 37 57 Graham Janet S. 37 57, Greater Glasgow & Clyde National Health Service Biankin Andrew V. 36 37 57 Chang David K. 36 37 57 Jamieson Nigel B. 37 57 Graham Janet S. 37 57 Oien Karen 37 57 Hair Jane 57, None, Whole genomes redefine the mutational landscape of pancreatic cancer. Nature. ,vol. 518, pp. 495- 501 ,(2015) , 10.1038/NATURE14169
Franck Bonnetain, Bert Bonsing, Thierry Conroy, Adelaide Dousseau, Bengt Glimelius, Karin Haustermans, François Lacaine, Jean Luc Van Laethem, Thomas Aparicio, Daniela Aust, Claudio Bassi, Virginie Berger, Emmanuel Chamorey, Benoist Chibaudel, Laeticia Dahan, Aimery De Gramont, Jean Robert Delpero, Christos Dervenis, Michel Ducreux, Jocelyn Gal, Erich Gerber, Paula Ghaneh, Pascal Hammel, Alain Hendlisz, Valérie Jooste, Roberto Labianca, Aurelien Latouche, Manfred Lutz, Teresa Macarulla, David Malka, Muriel Mauer, Emmanuel Mitry, John Neoptolemos, Patrick Pessaux, Alain Sauvanet, Josep Tabernero, Julien Taieb, Geertjan Van Tienhoven, Sophie Gourgou-Bourgade, Carine Bellera, Simone Mathoulin-Pélissier, Laurence Collette, None, Guidelines for time-to-event end-point definitions in trials for pancreatic cancer. Results of the DATECAN initiative (Definition for the Assessment of Time-to-event End-points in CANcer trials) ☆ European Journal of Cancer. ,vol. 50, pp. 2983- 2993 ,(2014) , 10.1016/J.EJCA.2014.07.011
Bastiaan BJ Tops, Nicola Normanno, Henriette Kurth, Eliana Amato, Andrea Mafficini, Nora Rieber, Delphine Le Corre, Anna Maria Rachiglio, Anne Reiman, Orla Sheils, Christoph Noppen, Ludovic Lacroix, Ian A Cree, Aldo Scarpa, Marjolijn JL Ligtenberg, Pierre Laurent-Puig, Development of a semi-conductor sequencing-based panel for genotyping of colon and lung cancer by the Onconetwork consortium BMC Cancer. ,vol. 15, pp. 26- 26 ,(2015) , 10.1186/S12885-015-1015-5
Marina Pasca Di Magliano, Craig D Logsdon, None, Roles for KRAS in Pancreatic Tumor Development and Progression Gastroenterology. ,vol. 144, pp. 1220- 1229 ,(2013) , 10.1053/J.GASTRO.2013.01.071
Nabeel Bardeesy, Ronald A. DePinho, Pancreatic cancer biology and genetics Nature Reviews Cancer. ,vol. 2, pp. 897- 909 ,(2002) , 10.1038/NRC949
Deniz Pekin, Yousr Skhiri, Jean-Christophe Baret, Delphine Le Corre, Linas Mazutis, Chaouki Ben Salem, Florian Millot, Abdeslam El Harrak, J Brian Hutchison, Jonathan W Larson, Darren R Link, Pierre Laurent-Puig, Andrew D Griffiths, Valérie Taly, None, Quantitative and sensitive detection of rare mutations using droplet-based microfluidics Lab on a Chip. ,vol. 11, pp. 2156- 2166 ,(2011) , 10.1039/C1LC20128J