Molecular characterization of canine parvovirus-2 variants circulating in Tunisia.

摘要: Canine parvovirus type 2 (CPV2) emerged in 1978 as a highly contagious and very serious disease dogs. The characterization of CPV2 antigenic types is exclusively based on the identification amino acid residue at position 426 capsid protein VP2. Currently, three CPV-2a (asparagine N426), CPV-2b (aspartic D426) CPV-2c (glutamic E426) are circulating worldwide. In Tunisia, despite fact that many clinical few serological investigations clearly indicate CPV widespread major concerns local dog population, no molecular variants has been carried out. This investigation showed most clinically presumed infections were confirmed by classical or real-time PCR. When PCR facilities affordable, reported here association with restriction fragment length polymorphism (RFLP) MboI MboII can be useful for screening diagnosing infections. A total 50 characterized sequencing an almost even representation different types, including slightly more 2b, evidenced. Characterization Tunisian MGB probe assays was inefficient because their typical nucleotide mutation C1269. Phylogenetic analysis underwent evolution relatively long period time inside country. also some crossings leaving both genotypic phenotypic characteristic mutations.