Structure of the rat interleukin 6 gene and its expression in macrophage-derived cells.

摘要: Rat peritoneal exudate cells produce two interleukin 6 (IL6) messenger RNA species, a major 1200 nucleotide and 5-fold less abundant, 2400-nucleotide species. A cDNA clone representing the species was isolated, sequenced. The 1055-base pair insert covered 3'-nontranslated region, 211 triplet coding region most of 5'-nontranslated region. derived rat IL6 amino acid sequence 93 58% identical, respectively, with mature murine human IL6. lacks N-glycosylation sites but contains fifth cysteinyl residue in addition to 4 residues shared conserved positions Stable L cell HeLa-derived lines were established by cotransfection selectable neomycin resistance marker. These secrete 9-fold increased amounts functional over their respective parental cells. stable macrophage-derived line, RM-SV1, transformation simian virus 40. Il1 mRNA levels are inducible 20- 3.5-fold, this line treatment lipopolysaccharide kinetics characteristic macrophages. set three overlapping genomic DNA clones isolated 10-kilobase segment sequenced containing gene plus 2.9 kilobases 5'-flanking 1.3 3'-flanking sequences. transcription start used RM-SV1 mapped within 5 base pairs each other. exon/intron boundaries genes. generated alternative polyadenylation at separated distance 1.2 kilobases. intervening repetitive element 72-80% identical consensus L1 family