Common proteomic changes in the hippocampus in schizophrenia and bipolar disorder and particular evidence for involvement of cornu ammonis regions 2 and 3.

摘要: Context: The hippocampus is strongly implicated in schizophrenia and, to a lesser degree, bipolar disorder. Proteomic investigations of the different regions may help us clarify basis and disease specificity changes. Objective: To determine whether disorder are associated with distinct patterns differential protein expression specific hippocampus. Design, Setting, Patients: A postmortem comparative proteomic study, including validation expression, was performed. Midhippocampus samples from well-matched groups 20 subjects schizophrenia, disorder, control cases Stanley Medical Research Institute Array Collection were analyzed. Main Outcome Measures: We used laser-assisted microdissection enrich for tissue hippocampal 2-dimensional difference gel electrophoresis compare profiles. Levels differentially expressed proteins confirmed by enzyme-linked immunosorbent assay Western blotting. Hippocampi haloperidol-treated mice discriminate drug-associated disease-associated Results: Across all regions, 108 spots 165 compared controls. Sixty-one both disorders. One hundred fifty-two these identified mass spectrometry, they range processes cytoskeletal metabolic functions. In disorders, cornu ammonis 2 3 affected significantly greater degree than other regions. Additionally, numerous showed changes more 1 region Validation work septin 11 involved clathrin-mediated endocytosis Conclusions: Overall, similar observed first time indicate that most prominent occur within 3. cellular trafficking process our study. Arch Gen Psychiatry. 2011;68(5):477-488