Evidence for new nuclear and mitochondrial genome organizations among high-frequency somatic embryogenesis-derived plants of allotetraploid Coffea arabica L. (Rubiaceae)

摘要: The most important commercial species of coffee, Coffea arabica, which produces 73% the world's coffee crop and almost all in Latin America, is only tetraploid (allotetraploid, 2n=4x=44) known genus. High-frequency somatic embryogenesis, plant regeneration recovery were achieved from leaf explants a mature, elite C. arabica cv. Cauvery (S-4347) using two-step culture method. To assess genetic integrity nuclear, mitochondrial chloroplast genomes among hardened regenerants, we employed multiple DNA markers (RFLP, RAPD, ISSR) for sampling various regions genome. Although nuclear mother five ramets derived ortet similar organization, this was not so embryo-derived plants where both changed different, characteristic ways produced novel genome organizations. A total 480 loci, based on data obtained 16 gene probes, combination with nine restriction enzyme digests, 38 RAPD 17 SSR primers, scored 27 single control. Among these, 44 loci observed to be polymorphic. relatively low level polymorphism (4.36%) found genome, while (41%) much higher. No detected 4 plants. Such selective true Thus, in-depth comprehensive study demonstrates, first time, presence subtle variability organizations commercially well-established embryogenesis-derived species.