Overexpression of microRNA-143 inhibits growth and induces apoptosis in human leukemia cells

摘要: Microrna-143 (miR-143) has been suggested to be a tumor suppressor, yet its role in hematological tumors not determined. Thus, we aimed explore the expression and function of miR-143 leukemia cells. was assessed bone marrow samples from 63 patients 15 healthy controls using q-PCR, correlation with DNMT3A In addition, after lentiviral-mediated overexpression, K562 cell proliferation evaluated CCK-8 analysis; cycle progression apoptosis were determined flow cytometry. The Bcl-2 pro-caspase-3 -9 by q-PCR western blot analysis, respectively. Leukemia had significantly lower relative than (P=0.004), levels miR143 DNMTA3A negatively correlated (r=-0.663, P=0.001). Overexpression decreased mRNA protein expression, reduced at 72 96 h (both P ≤ 0.018). colony formation observed upon overexpression. Flow cytometric analysis revealed that early rate higher group NC group. miR-143-expressing These findings suggest plays an important apoptosis, possibly through silencing DNMT3A. Further studies are necessary determine prognostic value therapeutic potential targeting miR-143.