Changes in iNOS, GFAP and NR1 Expression in Various Brain Regions and Elevation of Sphingosine-1-phosphate in Serum after Immobilized Stress

摘要: Several studies have been suggested that long-term exposure to stress has detrimental effects on various brain functions and leads neurodegenerative changes. However, the precise mechanism by which induces damage or change is still a matter of debate. This study investigated neuronal cells involving in expression iNOS, NR1, GFAP regions characterized sphingolipid metabolites as biomarker physiological serum after 3 weeks repeated immobilization. In this report, NR1 rats exposed chronic immobilization was investigated. The elevated cortex hippocampal area Immunoreactivity for vimentin, marker reactive gliosis, also hippocampus. level sphingolipids measured order assess changes stress. Interestingly, So-1-P increased plasma subjected 6-h than To further investigate modulating effect regions, infused into lateral cerebroventricle at rate 100 pmol/10 μl/h 7 days. iNOS cortex, hippocampus, striatum, cerebellum infusion cerebroventricle, while hippocampus striatum. levels GFAP, were direct application cultured cortical cells. These results suggest NO production via expression, activation astrocytes, elevation may cause would be one signal molecules.