T cell regulation of IgA immunoglobulin production in gut-associated lymphoid tissues

摘要: Abstract To explore mechanisms of gut-mucosal IgA immune response, we have established Con A-induced cloned T cell lines originating from PP and spleen. These cells expressed Thy-1.2+, Lyt-1+2−, Ia (I-A I-E) H-2 (K/D) surface antigens. Cloned derived were found to suppress LPS-induced IgM IgG synthesis secretion co-cultured B cells; in addition, whereas the did not bring about production, they cause appearance large numbers expressing sIgA. In contrast, spleen had little or no effect on by neither brought production nor studies provide evidence for existence a new type PP, switch cell, which is able induce undergo class-specific switches IgA; appear govern pathway DNA recombination (or RNA splicing) rather than cellular events resulting terminal differentiation. Thus, these are probably responsible fact that major source mucosal cells. additional studies, show post-switch cells, i.e. precultured with capacity differentiation into producing plasma provided exposed helper (uncloned) an appropriate mitogenic stimulus (staphylococcal protein A). We can conclude, therefore, development IgA-producing gut-associated lymphoid tissues (GALT) appears require at least two steps: one involves heavy chain switching governed outside (such as MLN spleen).