Sensitive plate assay for screening and detection of bacterial polyurethanase activity.
作者： G.T. Howard , J. Vicknair , R.I. Mackie
DOI: 10.1046/J.1472-765X.2001.00887.X
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摘要: G . T H O W A R D , J V I C K N M E 2001. 1 Aims: plate assay to screen and detect bacterial polyurethanase in agar medium containing a colloidal polyester-polyurethane rhodamine B is presented. Methods Results: Substrate hydrolysis causes the formation of orange fluorescent halos visible upon u.v. irradiation. The logarithm activity from purified protein linearly correlated with diameter halos, thereby allowing quantification activities ranging 0AE81 7AE29 Units. Conclusions: potential advantages this system are identification recovery viable polyurethanolytic bacteria activity. Significance Impact Study: These derived largely intense fluorescence observed due substrate reacting for use low concentrations corresponding decrease time required detecting levels enzyme
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