Recombinant JC viral DNA: Verification and physical map of prototype
作者： Jonathan D. Martin , Karl H. Brackmann , Brian W. Grinnell , Richard J. Frisque , Duard L. Walker
DOI: 10.1016/0006-291X(82)91567-4
关键词:
摘要: Abstract Genomic DNA of the human polyomavirus JC was molecularly cloned from extracted primary fetal glial cells infected with prototype (MAD-1) virus and diseased brain tissue which originally isolated. This report documents that these recombinant MAD-1 DNAs are identical, prototypical, may serve as unambiguous references to distinguish variant produced by independent isolates virus. Possible ambiguity in other recombinants is discussed. A new restriction endonuclease cleavage map derived for 52 sites cleaved eight enzymes.
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