Expression of a plant cystatin confers partial resistance to Globodera, full resistance is achieved by pyramiding a cystatin with natural resistance
作者： Peter E. Urwin , Jayne Green , Howard J. Atkinson
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摘要: Constructs based on a cysteine proteinase inhibitor (cystatin) from sunflower and protein engineered variant of rice cystatin (Oc-IΔD86) conferred similar levels resistance potato plants to Globodera spp as chicken egg white (CEWC) under the control CaMV35S. The level challenge by 6.5 viable eggs g–1 soil in small scale field trial was that provided natural partial cv. Sante. PCR an internal transcribed spacer region ribosomal cistron nematode genome established challenging population mixture G. pallida rostochiensis with no evidence differential effect transgenic reproductive success two species. Transformation Sante South American cultivar Maria Huanca CaMV35S/CEWC raised status both cultivars full this study. results establish potential plant cystatins demonstrate additive expressing CEWC genes.
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Robert M. Horton, Henry D. Hunt, Steffan N. Ho, Jeffrey K. Pullen, Larry R. Pease, Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene. ,vol. 77, pp. 61- 68 ,(1989) , 10.1016/0378-1119(89)90359-4
Stephen L. Dellaporta, Jonathan Wood, James B. Hicks, A plant DNA minipreparation: Version II Plant Molecular Biology Reporter. ,vol. 1, pp. 19- 21 ,(1983) , 10.1007/BF02712670
Howard J. Atkinson, Jayne Green, Sue Cowgill, Aurora Levesley, The case for genetically modified crops with a poverty focus Trends in Biotechnology. ,vol. 19, pp. 91- 96 ,(2001) , 10.1016/S0167-7799(00)01547-X
Peter E. Urwin, Michael J. McPherson, Howard J. Atkinson, Enhanced transgenic plant resistance to nematodes by dual proteinase inhibitor constructs Planta. ,vol. 204, pp. 472- 479 ,(1998) , 10.1007/S004250050281
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
Peter E. Urwin, Howard J. Atkinson, David A. Waller, Michael J. McPherson, Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida. Plant Journal. ,vol. 8, pp. 121- 131 ,(1995) , 10.1046/J.1365-313X.1995.08010121.X
Steffan N. Ho, Henry D. Hunt, Robert M. Horton, Jeffrey K. Pullen, Larry R. Pease, Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. ,vol. 77, pp. 51- 59 ,(1989) , 10.1016/0378-1119(89)90358-2
J. C. Veremis, P. A. Roberts, Relationships between Meloidogyne incognita resistance genes in Lycopersicon peruvianum differentiated by heat sensitivity and nematode virulence. Theoretical and Applied Genetics. ,vol. 93, pp. 950- 959 ,(1996) , 10.1007/BF00224098
Ronald E. Koes, Cornelis E. Spelt, Jos N. M. Mol, Anton G. M. Gerats, The chalcone synthase multigene family of Petunia hybrida (V30): sequence homology, chromosomal localization and evolutionary aspects Plant Molecular Biology. ,vol. 10, pp. 159- 169 ,(1987) , 10.1007/BF00016153
Guido Jach, Birgit Gornhardt, John Mundy, Jurgen Logemann, Elke Pinsdorf, Robert Leah, Jeff Schell, Christoph Maas, Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco Plant Journal. ,vol. 8, pp. 97- 109 ,(1995) , 10.1046/J.1365-313X.1995.08010097.X